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KMID : 0644120080210020166
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Journal of Oriental Gynecology
2008 Volume.21 No. 2 p.166 ~ p.183
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Anti-inflammatory Effects of Haedoksamultang in RAW 264.7 cells
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Kim Yang-Ho
Cho Han-Baek
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Abstract
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Purpose: This study was performed to determine anti-imflammatory effects of Haedoksamultang.
Methods: In this study, I examined the effects of Haedoksamultang on the production of nitric oxide(NO), tumor necrosis factor-α(TNF-α), and interlukin-1β(IL-1β) as well as the expression of inducible NO synthase(iNOS), cyclooxygenase-2(COX-2), TNF-α, and IL-1β in RAW 264.7 cells. Haedoksamultang inhibited LPS-stimulated NO production. Western blotting and RT-PCR analysis showed that Haedoksamultang suppressed LPS-induced iNOS and COX-2 protein and mRNA expression in RAW 264.7 cells. Haedoksamultang also suppressed the expression and production of LPS-stimulated TNF-α and IL-1β in RAW 264.7 cells. Haedoksamultang inhibited NF-κB activation in LPS-treated RAW 264.7 cells. Moreover, this compound blocked IκB-α phosphorylation and nuclear translocation of the cytosolic NF-κB p65 subunit, which highly correlated with the production and expression of inflammatory mediators.
Results: Haedoksamultang suppresses that inflammation-associated gene expression by blocking NF-κB activation.
Conclusion: These results suggest that Haedoksamultang may be beneficial for treating inflammatory disease.
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KEYWORD
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herbal medicine, anti-inflammatory effect, NO, TNF-α, IL-1β, NF-κB
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